Automated method for the determination of serum creatine kinase activity.
نویسنده
چکیده
An automated (AutoAnalyzer) method for the colorimetric determination of creatine kinase activity in serum is described. This method includes reactivation of creatine kinase with cysteine, incubation of the active enzyme with creatine phosphate and adenosine diphosphate at 3750, and subsequent inactivation of enzyme and binding of csteine by phenylmercuric borate. The enzymatically produced creatine is dialyzed against a solution of diacetyl and reacted with a-naphthol in an alkaline solution. The absorbance of the colored end product is measured at 550 mc. Individual blanks are determined in the absence of adenosine diphosphate. Comparison of results obtained by this method and a manual procedure shows satisfactory agreement.
منابع مشابه
Evaluation of cardiac troponin I (cTnI), electrocardiography, creatine kinase (CK) and aspartate aminotransferase (AST) in diagnosis of selenium deficiency in Markhoz goat kids breed
BACKGROUND: Cardiac Troponin I (cTnI) is known as a cardiac biomarker in determining the myocardial damage of diseases which affect the heart muscle. OBJECTIVES: This study aims to evaluate the serum cTnI concentration in Markhoz Breed goats suffering selenium (Se) deficiency and its correlation with electrocardiographic parameters, and activity of creatine kinase (CK) and aspartate aminotransf...
متن کاملThe effect of thirteen plants on arginase , creatine kinase, amylase and lipase activities of the serum in diabetic rats
Many traditional plants are used for treatment of diabetes throughout the world as adjuncts to conventional therapy. The purpose of this study was evaluate the effects of thirteen different plants on arginase, creatin kinase, amylase and lipase activities in serum of diabetic rats. Diabetes was induced in 70 adult male albino rats by intraperitoneal injection of 175 mg/ kg body weight of allox...
متن کاملThe effect of waterpipe smoking and chronic immobilization stress on serum creatine kinase and alkaline phosphatase activities in female rats
Background: Various studies indicate that there is association between life style and various enzyme activities. This study was conducted to determine the effects of waterpipe smoking and immobilization on serum creatine kinase and alkaline phosphatase activities in female rats. Methods: In this experimental study female Wistar rats were divided into control, waterpipe smoking, immobilized...
متن کاملComparison of Effect of Piperine and Capsaicin with Tabata Exercise on Changes in Serum Nitric Oxide and Creatine Kinase of Kung Fu Boys
Introduction: Tabata exercise programs can produce free radicals and muscle soreness and herbal supplements may be helpful as mediators in response to oxidative damage and muscle stress. Therefore, the purpose of this study was to compare the effects of these two supplements with Tabata exercise activity on nitric oxide and creatine kinase enzyme. Methods: The research was a quasi-experimental...
متن کاملIn Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility
Background Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase acti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 13 3 شماره
صفحات -
تاریخ انتشار 1967